TUBA1B antibody (AA 427-441)

Catalog No. ABIN303379

This Rabbit Polyclonal antibody specifically detects TUBA1B in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for TUBA1B antibody (AA 427-441) (ABIN303379)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBA1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-TUBA1B Antibody

Binding Specificity: AA 427-441

Specificity: This antibody makes an excellent Loading Control.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to Amino acids 427-441 of Human alpha Tubulin.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (2.5 μg/mL). Immunofluorescence. Western Blot (1/500-1/3000). ELISA (1/5000-1/25000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C. Dilute only prior to immediate use.

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: alpha Tubulin / TUBA1B

Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain

Gene ID: 10376

NCBI Accession: NP_006073

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase